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hek blue tm htlr 8 reporter cell lines  (InvivoGen)


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    Structured Review

    InvivoGen hek blue tm htlr 8 reporter cell lines
    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing <t>hTLR-8</t> were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.
    Hek Blue Tm Htlr 8 Reporter Cell Lines, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek blue tm htlr 8 reporter cell lines/product/InvivoGen
    Average 95 stars, based on 101 article reviews
    hek blue tm htlr 8 reporter cell lines - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Inhibitory Effects of Dietary N -Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine"

    Article Title: Inhibitory Effects of Dietary N -Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.00790

    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.
    Figure Legend Snippet: Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Techniques Used: Inhibition, Activation Assay, Expressing, Incubation, Spectrophotometry



    Similar Products

    hek blue tm htlr 8 reporter cell lines  (InvivoGen)
    95
    InvivoGen hek blue tm htlr 8 reporter cell lines
    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing <t>hTLR-8</t> were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.
    Hek Blue Tm Htlr 8 Reporter Cell Lines, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek blue tm htlr 8 reporter cell lines/product/InvivoGen
    Average 95 stars, based on 1 article reviews
    hek blue tm htlr 8 reporter cell lines - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Inhibitory Effects of Dietary N -Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine

    doi: 10.3389/fimmu.2020.00790

    Figure Lengend Snippet: Impact of the native, partially demannosylated and partially desialylated N -glycans on the inhibition of TLR-8 activation induced with ssRNA40 or R848. HEK293 cells expressing hTLR-8 were first incubated with (A) native N -glycans, (B) N -glycans treated with mannosidase, and (C) N -glycans treated with sialidase at 4 mg/mL ( n = 5). After 1 h, the TLR-8 agonists ssRNA40 (50 μg/mL) and R848 (100 μg/mL) were used to stimulate the cells. NF-κB activation was measured by spectrophotometry at 650 nm. Data is represented as mean ± SEM. Statistical differences were measured using paired t -test. Significant differences compared to ssRNA40 and R848 are indicated by * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Article Snippet: HEK-Blue TM hTLR-8 reporter cell lines and reagents, such as selection media Quanti-Blue TM reagent, and the agonists single-stranded GU-rich oligonucleotide complexed with LyoVec (ssRNA40/LyoVec TM ) and R848 (resiquimod), were purchased from InvivoGen (Toulouse, France).

    Techniques: Inhibition, Activation Assay, Expressing, Incubation, Spectrophotometry